mt1 mt2 antagonists  (Millipore)

Journal:

Article Title: EXACERBATED MECHANICAL ALLODYNIA IN RATS WITH DEPRESSION-LIKE BEHAVIOR

doi: 10.1016/j.brainres.2008.01.038

Figure Lengend Snippet: A: When examined on postoperative day 7, the immobility time in the forced swimming test (FST) in WKY rats was significantly improved by melatonin (MT, 4 µg, once daily × 7 days) administered into the ACC contralateral to CCI. The melatonin effect was blocked by the broad MT receptor antagonist luzindole [10 µg but not 5 µg (L10, L5)] injected into the same ACC at 10 min before melatonin (4 µg). *P<0.05, as compared with the sham-vehicle (V) group; ++ P<0.01, as compared with the CCI-vehicle group; ## P<0.01, as compared with the CCI-MT group. B. The same melatonin (MT) treatment also improved the immobility time in the horizontal suspension test (HST) in WKY rat on day 7 after CCI. *P#0.05, as compared with sham and CCI-MT groups.

Article Snippet: Melatonin and luzindole (a broad MT receptor antagonist; MT2 > MT1) were purchased from Sigma (St. Louis, MO) and dissolved in 5% ethanol diluted in artificial cerebral spinal fluid (CSF) containing (in mM) NaCl 126, KCl 2.5, NaHCO 3 26, CaCl 2 2, MgCl 2 2, NaH 2 PO 4 1.25, glucose 10 (pH 7.35).

Techniques: Injection

Journal:

Article Title: EXACERBATED MECHANICAL ALLODYNIA IN RATS WITH DEPRESSION-LIKE BEHAVIOR

doi: 10.1016/j.brainres.2008.01.038

Figure Lengend Snippet: A, B: Exacerbation of mechanical allodynia was significantly improved in WKY rats by administration of melatonin (MT, 4 µg, once daily × 7 days) into the ACC contralateral to CCI. Melatonin alone did not affect the behavioral response in WKY rats with sham operation (P>0.05). ** P< 0.01, as compared with CCI-MT groups. The melatonin effect was blocked by luzindole (Lu) injected into the contralateral ACC at 10 min before melatonin (4 µg). *P<0.05, **P<0.01, as compared with CCI-MT groups. C: The effect of melatonin (MT) was dose-dependent (4 > 2 µg =vehicle) in WKY rats and melatonin (2 or 4 µg) did not change mechanical allodynia in Wistar rats after CCI. * P< 0.05 and ** P<0.01, as compared to Wistar rats.

Article Snippet: Melatonin and luzindole (a broad MT receptor antagonist; MT2 > MT1) were purchased from Sigma (St. Louis, MO) and dissolved in 5% ethanol diluted in artificial cerebral spinal fluid (CSF) containing (in mM) NaCl 126, KCl 2.5, NaHCO 3 26, CaCl 2 2, MgCl 2 2, NaH 2 PO 4 1.25, glucose 10 (pH 7.35).

Techniques: Injection

Journal:

Article Title: EXACERBATED MECHANICAL ALLODYNIA IN RATS WITH DEPRESSION-LIKE BEHAVIOR

doi: 10.1016/j.brainres.2008.01.038

Figure Lengend Snippet: The basal MT1 receptor mRNA (A: real-time PCR) and protein level (B: Western blot; C: statistics) in the contralateral ACC was lower in WKY than in Wistar rats. There were no significant differences in the basal MT2 receptor mRNA and protein level in the contralateral ACC between WKY and Wistar rats. A, C: *P<0.05, as compared to WKY rats. Rs-ACC and Ys-ACC: samples taken from the contralateral ACC of Wistar and WKY rats, respectively.

Article Snippet: Melatonin and luzindole (a broad MT receptor antagonist; MT2 > MT1) were purchased from Sigma (St. Louis, MO) and dissolved in 5% ethanol diluted in artificial cerebral spinal fluid (CSF) containing (in mM) NaCl 126, KCl 2.5, NaHCO 3 26, CaCl 2 2, MgCl 2 2, NaH 2 PO 4 1.25, glucose 10 (pH 7.35).

Techniques: Real-time Polymerase Chain Reaction, Western Blot

Journal:

Article Title: EXACERBATED MECHANICAL ALLODYNIA IN RATS WITH DEPRESSION-LIKE BEHAVIOR

doi: 10.1016/j.brainres.2008.01.038

Figure Lengend Snippet: A, B: The MT1 (A) and MT2 (B) receptor expression (real-time PCR) in the contralateral ACC was downregulated after CCI in both WKY and Wistar rats. However, the expression of MT1 and MT2 receptors in WKY rats was significantly lower than that in Wistar rats when examined on postoperative day 3 (MT2) and day 7 (MT1 & MT2). *P<0.05, as compared to day 0 in each corresponding group. # P<0.05, as compared to Wistar rats on the same day.

Article Snippet: Melatonin and luzindole (a broad MT receptor antagonist; MT2 > MT1) were purchased from Sigma (St. Louis, MO) and dissolved in 5% ethanol diluted in artificial cerebral spinal fluid (CSF) containing (in mM) NaCl 126, KCl 2.5, NaHCO 3 26, CaCl 2 2, MgCl 2 2, NaH 2 PO 4 1.25, glucose 10 (pH 7.35).

Techniques: Expressing, Real-time Polymerase Chain Reaction